Influences of Oldenlandia diffusa on the CYP450 Activities in Rats Using a Cocktail Method by UHPLC-MS/MS

Table 2

The pharmacokinetics parameters of phenacetin, omeprazole, tolbutamide, midazolam, and metoprolol in rat plasma after administration of oldenlandia diffusa.

Probe drug	Pharmacokinetics parameters	Control group	Oldenlandia diffusa-treated group
Phenaceton	AUC (0–t) (mg/L∗h)	6.00 ± 1.60	6.28 ± 1.81
	AUC (0–∞) (mg/L∗h)	7.37 ± 2.12	13.1 ± 9.31
	t1/2 (h)	5.98 ± 3.58	24.67 ± 39.93
	Tmax (h)	0.22 ± 0.14	0.17 ± 0.00
	Vz/F (L/kg)	23.3 ± 10.5	34.6 ± 34.8
	CLz/F (L/h/kg)	2.90 ± 0.80	1.98 ± 0.82
	Cmax (mg/L)	5.78 ± 1.66	5.72 ± 0.76
Omeprazole	AUC (0–t) (mg/L∗h)	1.45 ± 0.522	1.63 ± 0.754
	AUC (0–∞) (mg/L∗h)	1.48 ± 0.528	1.64 ± 0.759
	t1/2 (h)	3.28 ± 1.47	1.86 ± 1.08
	Tmax (h)	0.17 ± 0.00	0.17 ± 0.00
	Vz/F (L/kg)	77.8 ± 56.0	33.2 ± 12.0
	CLz/F (L/h/kg)	16.1 ± 9.20	15.5 ± 9.03
	Cmax (mg/L)	2.80 ± 0.862	3.38 ± 1.60
Tolbutamide	AUC (0–t) (mg/L∗h)	60.2 ± 22.7	58.2 ± 22.3
	AUC (0–∞) (mg/L∗h)	61.9 ± 23.7	62.5 ± 26.3
	t1/2 (h)	4.21 ± 0.69	5.32 ± 1.84
	Tmax (h)	2.00 ± 1.67	2.27 ± 1.21
	Vz/F (L/kg)	0.11 ± 0.03	0.13 ± 0.05
	CLz/F (L/h/kg)	0.02 ± 0.01	0.02 ± 0.01
	Cmax (mg/L)	5.29 ± 1.78	5.45 ± 2.29
Midazolam	AUC (0–t) (mg/L∗h)	8.24 ± 8.25	13.8 ± 9.57
	AUC (0–∞) (mg/L∗h)	36.1 ± 83.0	77.9 ± 81.7
	t1/2 (h)	29.3 ± 63.4	60.0 ± 68.3
	Tmax (h)	0.88 ± 0.82	0.17 ± 0.00
	Vz/F (L/kg)	28.0 ± 17.2	44.1 ± 80.2
	CLz/F (L/h/kg)	4.93 ± 4.69	3.42 ± 4.44
	Cmax (mg/L)	2.00 ± 0.608	2.38 ± 0.641
Metoprolol	AUC (0–t) (mg/L∗h)	3.33 ± 0.798	3.16 ± 0.576
	AUC (0–∞) (mg/L∗h)	3.54 ± 1.05	3.94 ± 1.53
	t1/2 (h)	6.80 ± 5.09	15.7 ± 18.5
	Tmax (h)	0.920 ± 0.510	0.690 ± 0.630
	Vz/F (L/kg)	53.9 ± 30.8	89.7 ± 79.3
	CLz/F (L/h/kg)	6.06 ± 1.58	5.72 ± 1.95
	Cmax (mg/L)	0.865 ± 0.142	0.933 ± 0.172