Postrecruitment Function of Yeast Med6 Protein during the Transcriptional Activation by Mediator Complex

Figure 2

Schematic presentations of Med6p derivatives and summary of their functional defects in vivo and in vitro. Viability¹ was tested for the ability of deletion mutants to complement the med6 null mutation via plasmid shuffling as described in Materials and Methods. In vitro activity² represents the capability of each recombinant form of Med6 proteins to rescue the in vitro transcriptional defects of med6-ts2 holoenzyme based on the quantitative data shown in Figure 4. β-galactosidase activity³ indicates the expression levels of the reporter gene in the artificial recruitment assay of the indicated Med6p derivatives as shown in Figure 6(a).