The Binding Affinity of Small Molecules with Yam Tyrosinase (Catechol Oxidase): A Biophysical Study

Figure 2

Binding sensogram for compounds having interactions with immobilized yam (Amorphophallus paeoniifolius) tyrosinase. Flow rate was maintained at 45 μL/min. Contact time and dissociation time were kept at 120 s and 200 s. Regeneration was carried out using 10 mM glycine HCl at pH 2.5 for 30 s and at 30 μL/min. The data analysis was performed with Biacore X100 evaluation software version 2.0.1, and data were fit to two states. Each concentration represents sensorgram in the increasing order of curves. Crocin with concentrations (0.156, 0.312, 0.625, 1.25, and 2.5 mM) was injected over the enzyme surface (a). Kojic acid with concentrations (0.0156, 0.0312, 0.0625, 0.125, and 0.25 mM) was injected over the enzyme surface (b). Hydroquinone with concentrations (0.625, 1.25, 2.5, 5.0, and 10 mM) was injected over enzyme surface (c). L-DOPA with concentrations (5, 10, 20, 40 and 80 mM) was injected over the enzyme surface (d).

(a)

(b)

(c)

(d)