The Binding Affinity of Small Molecules with Yam Tyrosinase (Catechol Oxidase): A Biophysical Study

Figure 3

Binding sensogram for cocktail interactions with immobilized yam (Amorphophallus paeoniifolius) tyrosinase. Flow rate was maintained at 45 μL/min. Contact time and dissociation time were kept at 120 s and 200 s. Regeneration was carried out using 10 mM glycine HCl at pH 2.5 for 30 s and at 30 μL/min. The data analysis was performed with Biacore X100 evaluation software version 2.0.1, and data were fit to a heterogenous analyte model. Each concentration represents sensorgram in the increasing order of curves. Concentrations of hydroquinone (0.625, 1.25, 2.5, 5.0, and 10 mM) + crocin (0.156, 0.312, 0.625, 1.25, and 2.5 mM) were injected over the enzyme surface (a). Concentrations of kojic acid (0.0156, 0.0312, 0.0625, 0.125, and 0.25 mM) + hydroquinone (0.625, 1.25, 2.5, 5.0, and 10 mM) were injected over the enzyme surface (b). Concentrations of crocin (0.156, 0.312, 0.625, 1.25, and 2.5 mM) + L-DOPA (5, 10, 20, 40, and 80 mM) were injected over the enzyme surface (c).

(a)

(b)

(c)