Research Article
The Binding Affinity of Small Molecules with Yam Tyrosinase (Catechol Oxidase): A Biophysical Study
Table 2
Interaction kinetics of yam tyrosinase with a mixture of two different inhibitors fit to a heterogeneous analyte model.
| | Compounds | ka1 (1/ms) | kd1 (1/s) | ka2 (1/s) | kd2 (1/s) | KD1 (M) | KD2 (M) |
| | Hydroquinone + crocin | 32.99 × 104 ± 3.2 × 104 | 0.0705 ± 2.4 × 10−3 | 5.93 × 104 ± 7.80 × 103 | 4.24 × 10−2 | 2.14 × 10−7 | 7.15 × 10−7 | | Kojic acid + hydroquinone | 6.36 × 104 ± 26 × 103 | 0.03372 ± 2.3 × 10−3 | 6.7 × 104 ± 2.0 × 104 | 1.51 × 10−1 | 5.31 × 10−7 | 2.26 × 10−6 | | Crocin + L-DOPA | 6.62 × 103 ± 7.6 × 103 | 0.001587 ± 0.3 × 10−3 | 11.05 × 104 ± 1.2 × 105 | 2.54 × 10−2 | 2.4 × 10−7 | 2.3 × 10−7 |
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KD1 and KD2 represent rate constants of first and second inhibitors in a cocktail, respectively.
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