miR-142-5p as a CXCR4-Targeted MicroRNA Attenuates SDF-1-Induced Chondrocyte Apoptosis and Cartilage Degradation via Inactivating MAPK Signaling Pathway

Figure 3

miR-142-5p overexpression attenuated SDF-1-induced chondrocyte apoptosis by targeting CXCR4. (a–e) Primary human OA chondrocytes were transfected with the indicated miRNA mimics and plasmids. At 48 h after transfection, cells were then treated with SDF-1 (100 ng/ml) for another 24 h. (a-b) Chondrocyte apoptosis was assessed by flow cytometry. The representative flow profilings of Annexin-V and PI staining are shown (a), and the rates of apoptosis in primary human OA chondrocytes were summarized (b). (c)The mRNA levels of Bax and Bcl-2 were determined with RT-qPCR. (d-e) The expression levels of cleaved caspase-3, cleaved PARP, Bax and Bcl-2 were determined by Western blot assays. The representative bands images are shown (d), and relative bands intensity was summarized (e). Data are presented as the mean ± SEM (n = 3). ; versus the miR-NC + vector group; and versus the miR-142-5p mimic + vector group. miRNAs, microRNAs; SDF-1, stromal cell-derived factor 1; NC, negative control; Bcl-2, B-cell lymphoma-2; cleaved PARP, cleaved poly ADP-ribose polymerase; Gapdh, glyceraldehyde‐3‐phosphate dehydrogenase.

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