Interaction of Agaric Acid with the Adenine Nucleotide Translocase Induces Mitochondrial Oxidative Stress

Figure 1

(a) Agaric acid effect on the matrix Ca²⁺ content release. Mitochondrial protein (2 mg) was added to 3 ml of a basic medium as described in Materials and Methods. Where indicated, 3 μM agaric acid (Ag Ac), 2 µM cyclosporin A (CSA), and 20 µM tamoxifen (TAM) were added. Traces are representative of, at least, 4 different experiments. (b) Agaric acid-induced collapse of the transmembrane electric gradient. Mitochondrial protein was incubated in 3 ml of a medium. Where indicated, 50 µM CaCl₂, 3 µM agaric acid, 20 µM tamoxifen, and 1 µM of the uncoupler CCCP were added. (c) Attenuation by tamoxifen of the agaric acid-induced mitochondrial swelling. Mitochondrial protein was incubated in 3 ml of basic. Where indicated, 50 µM CaCl₂, 3 µM agaric acid, and 20 µM tamoxifen were added. Temperature: 25°C. Traces are representative of, at least, 4 different experiments.

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