Comparison between Conventional Decalcification and a Microwave-Assisted Method in Bone Tissue Affected with Mycetoma

Table 2

Decalcifying solution scores as the measurement of decalcification time and morphological fungi preservation.

Decalcifying solutions

Decal/time; RT/MW; Hours/minutes

M. mycetomatis fungi morphological evaluation

Total score

FSHE, RT/MW

FSG, RT/MW

FSGr, RT/MW

P

F

G

E

P

F

G

E

P

F

G

E

10% EDTA

120 h/29 h: 4 min

3/1

5/2

10/4

32/43

1/5

1/7

15/6

33/32

1/0

1/0

5/9

43/41

50/50

5% nitric acid

7 h: 30min/3:45 min

2/3

2/3

37/2

9/42

5/3

4/4

8/4

33/39

1/3

2/4

15/9

32/34

50/50

5% HCl

8 h/5 h: 30 min

2/2

5/4

42/11

1/33

5/1

5/1

18/17

22/31

5/2

4/2

18/11

23/35

50/50

Chi-square test

value: 0.023

value: 0.001

value: 0.03

Note. Decal: decalcification. FSHE: fungi staining with haematoxylin and eosin. FSG: fungi staining with Gridley stain. FSGr: fungi staining with Grocott hexamine-silver stain. RT: room temperature. MW: microwave oven. P: poor. F: fair. G: good. E: excellent.