Activity of Phosvitin in Hydroxyapatite Acid-Damage Immersion and Antimicrobial Assays

Figure 4

Effect of addition of phosvitin on calcium release form hydroxyapatite discs exposed to the month-long acid-damage immersion assay. Discs were treated with phosvitin solutions ranging from 0.13 to 1.0 mg/mL phosvitin. FAAS measurements of the calcium released into solution were taken at the initial onset of the experiment and also after thirty days. A correction for calcium contributed by the phosvitin solution was made by subtracting the initial time measurement from that after thirty days. Inset: standard curve relating calcium concentration to FAAS signal; error measurements were calculated but were inside the span of the markers.