Isolation, Extraction, Purification, and Molecular Characterization for Thermostable α-Amylase from Locally Isolated Bacillus Species in Sudan

Table 1

Partial purification steps of α-amylase enzyme from Bacillus cereus.

Purification step	Volume	Total protein (mg/ml)	Total activity (U/ml)	Specific activity (U/mg)	Purification fold	Yield%
Crude	82	75.3	15.959	0.211	1	100
(0–30%)	5	4.04	1.617	0.400	1.89	10.13
(30–80%)	25	1.8368	0.0317	0.0172	0.081	0.198