Isolation, Extraction, Purification, and Molecular Characterization for Thermostable α-Amylase from Locally Isolated Bacillus Species in Sudan

Table 2

Partial purification steps of α-amylase from Bacillus licheniformis.

Purification step	Volume	Total protein (mg/ml)	Total activity (U/ml)	Specific activity (U/mg)	Purification fold	Yield%
Crude	100	52.20	247.20	4.736	1.00	100
(0–30%)	9.5	5.91	13.17	2.228	0.47	5.33
(30–80%)	9.5	8.19	12.31	1.503	0.32	4.98
Dialysis	6.8	1.61	8.47	5.261	1.11	3.43