Diverse Effect of Vitamin C and N-Acetylcysteine on Aluminum-Induced Eryptosis

Figure 4

The effect of vit C and NAC on Al-induced PS externalization using Annexin V-PE flowcytometric methods. (a) Histogram of PS externalization of erythrocytes following exposure for 24 hours to Ringer solution (control; grey area), AlCl₃ (100 μM; black line), AlCl₃ (100 μM) and vit C (0.6 mM; dashed line), AlCl₃ (100 μM), and NAC (1.0 mM; dotted line). (b) Arithmetic means ± SD of the percentage of erythrocyte with enhanced PS externalization following treatment with AlCl₃ in the presence and absences of vit C (0.6 mM) and NAC (1 mM) in Ringer solution for 24 hours. and  < 0.05 significant difference compared to the AlCl₃-treated group.

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