Hypoxia-Induced miR-210 Promotes Endothelial Cell Permeability and Angiogenesis via Exosomes in Pancreatic Ductal Adenocarcinoma

Figure 2

The identification of miR-210 in PDAC exosomes. (a) Heatmap of the screened miRNAs of exosomes from Hs766T and Hs766T-L2 cells. The fold change in each screened miRNA is attached in the right column. (b) Relative miR-210 expression in exosomes of Hs766T and Hs766T-L2 cells was measured by qRT-PCR. (c) Relative miR-210 expression in the indicated PDAC or normal cell lines was measured by qRT-PCR. (d) Relative miR-210 expression in the indicated HUVECs was measured by qRT-PCR. (e) Relative miR-210 expression in the indicated exosomes of HUVECs was measured by qRT-PCR. (f) Relative HIF-1α expression in the indicated HUVECs was measured by WB analysis. (g) Relative expression of miR-210 in the indicated treated cells was measured by qRT-PCR. +miR-210, miR-210 was overexpressed by miR-210 lentivirus; −miR-210, miR-210 was inhibited by miR-210 shRNA.

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