Hypoxia-Induced miR-210 Promotes Endothelial Cell Permeability and Angiogenesis via Exosomes in Pancreatic Ductal Adenocarcinoma

Figure 6

miR-210 or EFNA3 participates in the PI3K/AKT/VEGFA or Wnt/Β-catenin/RHOA pathways. (a) Relative indicated protein expression in the indicated HUVECs was measured by WB analysis. (b) Dil-labeled exosomes were added to Calcein AM-labeled HUVECs for cocultivation. Images were captured with a fluorescence microscope, and the white arrows indicate exosomes. Magnification, ×100. (c) Dio-labeled exosomes of ov cy5-miR-210 Hs766T were added to HUVECs for cocultivation. Images were captured with a fluorescence microscope. The green arrow indicates exosomes, and the pink arrow indicates cy5-miR signals. Magnification, ×200.

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