Bacterial Metabolomics: Sample Preparation Methods

Table 3

Summary on comparison between centrifugation and fast filtration method for cell harvesting.

	Centrifugation	Fast filtration
Feasibility	(i) More convenient.	(i) Fast processing rate.
	(ii) Reduce variability between sample.	(ii) No or very minimal metabolite leakage.
	(iii) Can be at high cell concentration.	(iii) Instantaneous removal of culture media.
	(iv) Can be subjected to instantaneous quenching
Disadvantages	(i) Longer processing time.	(i) Only at low cell concentration to avoid filter blockage.
	(ii) May induce physiological stress and metabolites leakage.	(ii) Unsuitable for high turnover rate metabolites.
	(iii) Unsuitable for certain bacteria, e.g., S. aureus.	(iii) Requires optimization step.
		(iv) High variability of extraction variation.