miR-21 Regulates Immune Balance Mediated by Th17/Treg in Peripheral Blood of Septic Rats during the Early Phase through Apoptosis Pathway

Figure 8

The levels of IL-17 and IL-10 in peripheral blood were changed after transfection of miR-21 activator or inhibitor into lymphocytes of septic rats. ((a) and (b)) The levels of IL-17 and IL-10 in peripheral blood in eight-paired NC rat group, SP rat group, SP rat + miR21 mimic group, and SP rat + miR21 inhibitor group were determined by ELISA [t value (IL-17: NC versus SP) = 13.660,  < 0.01; t value (IL-17: miR-21 mimic versus SP) = 5.096,  < 0.01; t value (IL-17: miR-21 inhibitor versus SP) = 8.638,  < 0.01; t value (IL-10: NC versus SP) = 29.703,  < 0.01; t value (IL-10: miR-21 mimic versus SP) = 18.042,  < 0.01; t value (IL-10: miR-21 inhibitor versus SP) = 14.523,  < 0.05]. NC group: normal control group; SP group: sepsis group; SP rat + miR21 mimic group: sepsis group with miR-21 activator transfected into lymphocytes; SP rat + miR21 inhibitor group: sepsis group with miR-21 inhibitor transfected into lymphocytes.  < 0.01.

(a)

(b)