Table of Contents
Biotechnology Research International
Volume 2011, Article ID 838232, 8 pages
http://dx.doi.org/10.4061/2011/838232
Research Article

Applicability of Three Alternative Instruments for Food Authenticity Analysis:GMO Identification

Molecular and Cell Biology, LGC, Queens Road, Teddington, Middlesex TW11 0LY, UK

Received 6 July 2010; Revised 22 November 2010; Accepted 19 December 2010

Academic Editor: Yves Bertheau

Copyright © 2011 A. Burrell et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Ensuring foods are correctly labelled for ingredients derived from genetically modified organisms (GMOs) is an issue facing manufacturers, retailers, and enforcement agencies. DNA approaches for the determination of food authenticitys often use the polymerase chain reaction (PCR), and PCR products can be detected using capillary or gel electrophoresis. This study examines the fitness for purpose of the application of three laboratory electrophoresis instruments (Agilent Bioanalyzer 2100, Lab901 TapeStation, and Shimadzu MCE-202 MultiNA) for the detection of GMOs using PCR based on a previously validated protocol. Whilst minor differences in the performance characteristics of bias and precision were observed, all three instruments demonstrated their applicability in using this protocol for screening of GMO ingredients.