High-Frequency Regeneration of the Drought-Tolerant Tree Melia volkensii Gurke Using Low-Cost Agrochemical Thidiazuron

Table 1

Effect of TDZ concentration on the induction of callus and somatic embryogenesis from mature zygotic embryos of M. volkensii.

TDZ concentration; (mg/L)	Total explants	% callus induction^†; (mean ± SE)	Fresh mass per callus (mg)^†; (mean ± SE)	% of callus with somatic embryos^††; (mean ± SE)
0	45	100 ± 0.00^a	156.44 ± 40.45^b	0^a
0.05	45	100 ± 0.00^a	304.87 ± 22.22^a	96.67 ± 3.34^b
0.125	45	100 ± 0.00^a	124.67 ± 16.65^b	90.00 ± 10.00^b
0.25	45	100 ± 0.00^a	157.11 ± 17.97^b	76.67 ± 3.34^b
0.50	45	100 ± 0.00^a	159.67 ± 45.86^b	70.00 ± 3.33^b
1.0	45	100 ± 0.00^a	122.48 ± 26.30^b	60.00 ± 6.67^b
2.0	45	100 ± 0.00^a	111.33 ± 22.04^b	53.33 ± 0.00^c
4.0	45	96.67 ± 3.34^a	100.11 ± 23.38^b	33.33 ± 16.67^ac

^†Data recorded after 21 days of culture on B5 medium containing TDZ. ^††Data obtained 30 days after transfer to hormone-free B5 medium (𝑛=30). Values with the same superscript do not differ significantly using Tukey’s HSD test at 𝑃≤0.05.