Plasminogen Activator Inhibitor-Type I Gene Deficient Mice Show Reduced Influx of Neutrophils in Ventilator-Induced Lung Injury

Figure 1

(a) Plasminogen activator inhibitor- (PAI-) 1 mRNA in lung. PAI-1 mRNA as determined by radioactive in situ hybridization (autoradiographic film). (b) PAI-1 levels in bronchoalveolar lavage fluid in anesthetized nonventilated control (c) mice, mice ventilated with low tidal volumes (LV_T) and high V_T (HV_T). Levels of PAI-1 in PAI-1^−/− mice were undetectable. Data are represented as individual data with a median. In situ hybridization for murine PAI-1 mRNA was performed on paraffin slides of nonventilated control Wt mice (c), Wt mice ventilated with LV_T (d) and HV_T (e) for 5 hours. Positive signal in black. Original magnification 100x.

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