Manipulation of the Complement System for Benefit in Sepsis

Table 2

Protective effects of anti-C5a rabbit IgG in CLP rats.

Type of antibody	Time for antibody infusion (hr)**	Survival at 7 days*
		Anti-C5aM	Anti-C5aC	Preimmune IgG
Rabbit IgG (600 μg i.v.)	0	90%	85%	30%
Rabbit IgG (600 μg i.v.)	6	70%	60%	35%
Rabbit IgG (600 μg i.v.)	12	50%	40%	17%

*Expressed as percent of all rats in each group (𝑛≥8). The M region of rat C5a involved amino acid residues 17–36 while the C region involved residues 58–77. Peptides were covalently linked to keyhole limpet hemocyanin followed by immunizations of rabbits. Antibodies were isolated by antigen affinity purification. Adapted from data in [13].
**Time (hr) of antibody infusion after CLP.