Characterization of Bacterial Etiologic Agents of Biofilm Formation in Medical Devices in Critical Care Setup

Table 3

Screening of 100 bacterial isolates for biofilm formation by microtitre plate method in different media and at 16, 20, and 24 hr incubation periods.

	No. of isolates
Biofilm formation (OD492-630mm)	TSB			TSB, 0.25% glucose			TSB, 0.5% glucose
	16 hr	20 hr	24 hr	16 hr	20 hr	24 hr	16 hr	20 hr	24 hr
High (ODc < OD > 2 × ODc)	1	1	1	1	2	1	2	1	2
Moderate (2 × ODc < OD = 4 × ODc)	17	24	19	19	18	17	15	21	17
Weak (ODc < OD > 2 × ODc)	39	43	44	44	49	43	33	32	30

Experiment was done in quadruplet and repeated two times. All OD_492-630mm values were expressed as average with standard deviation.