Bazedoxifene Plays a Protective Role against Inflammatory Injury of Endothelial Cells by Targeting CD40

Figure 1

Bazedoxifene plays a protective effect against TNF-α-induced VEC inflammation injury and the adhesion between THP-1 and VECs. (a) TNF-α decreased VEC viability in a dose-dependent manner when the concentration was higher than 25 ng/mL. (b) Treatment of VECs with different concentration bazedoxifene for 24 h significantly decreased the percentage of viable cells when the concentration was higher than 4 μmol/L. (c) Pretreatment with bazedoxifene at 4 μmol/L prevented TNF-α-induced damage on VECs. (d) THP-1 was labeled with BCECF-AM for 30 min and then added to EVCs for coculture for another 30 min. The images were captured by Operetta High Content Imaging System. Scale bar: 200 μm. (e) The expressions of VCAM-1 and ICAM-1. All values are expressed as the (); and , compared with the control group; ^##, compared with the TNF-α group.

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