Low Ethanol Concentrations Promote Endothelial Progenitor Cell Capacity and Reparative Function

Figure 6

Validation of ethanol-dependent expression of VEFG, PlGF, sFlt-1, sEng, and VE-cadherin. Gene expression levels, protein expression, and secreted protein levels of ECFC from untreated ECFC or after treatment with 1% ethanol were determined. For each experimental group, triplicates were created and RT-PCR runs (a), immunoblot (b, d), and ELISA (c) were performed for each ECFC cell line. Results represent of at least 6 independent experiments.

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