Long Noncoding RNA HOTAIR Functions as a Competitive Endogenous RNA to Regulate Connexin43 Remodeling in Atrial Fibrillation by Sponging MicroRNA-613
Two-step luciferase assays confirmed HOTAIR may directly competing with GJA1 for miR-613 binding and positively regulate the Cx43 expression by sponging miR-613 as a ceRNA. (a) The binding site of miR-613 and GJA1 3UTR. (b) GJA1 is a direct downstream target of miR-613; , against the GJA1 WT + miR-613 NC group. (c) HOTAIR may directly competing with GJA1 for miR-613 binding; , against the miR-613 NC group; #, against the miR-613 mimic group. miR: microRNA; HOTAIR: HOX transcript antisense RNA; UTR: untranslated regions; ceRNA: competitive endogenous RNA. The error bars mean standard deviation.
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