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Cardiovascular Therapeutics
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Cardiovascular Therapeutics
/
2020
/
Article
/
Tab 1
/
Research Article
Av
β
3 Single-Stranded DNA Aptamer Attenuates Vascular Smooth Muscle Cell Proliferation and Migration via Ras-PI3K/MAPK Pathway
Table 1
PCR reaction system.
Component
Volume
ssDNA (1
μ
M)
1
μ
L
av
β
3-F (10
μ
M)
1
μ
L
av
β
3-R (10
μ
M)
1
μ
L
2x PCR mix
10
μ
L
ddH
2
O (DNase-free)
To 20
μ
L
Total volume
20
μ
L