Epigenetic Modulation by Apabetalone Counters Cytokine-Driven Acute Phase Response In Vitro, in Mice and in Patients with Cardiovascular Disease

Figure 2

Apabetalone downregulates cytokine-induced expression of APR genes in hepatocytes. (a, b) Apabetalone (25 μM) reduces IL-6 mediated induction (72 h) of CRP, A2M, and APCS mRNA (rtPCR) (a) and protein (ELISA) (b). Data is presented as the (c, d) Apabetalone (25 μM) reduces IL-1β-mediated induction of CRP mRNA (c) and protein (d). Data is presented as the (e) Hepatic APR gene expression is downregulated by apabetalone in the LPS mouse model of systemic inflammation (rtPCR, 24 h post-LPS injection). (f) Innate immune gene expression in mouse liver (real-time PCR, 24 h post-LPS injection). (e, f) Data acquired from 6 to 8 animals per group and presented as a normalized to naïve mice. Statistical significance was determined through 1-way ANOVA analysis followed by Tukey’s multiple comparison test, where , , , and ns means nonsignificant.

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