Ginkgolide B Protects Cardiomyocytes from Angiotensin II-Induced Hypertrophy via Regulation of Autophagy through SIRT1-FoxO1

Figure 2

Effect of Ginkgolide B on autophagy in Ang II-induced cardiomyocytes. (a) Fluorescence microscopy of H9c2 cells staining with an LC3-II antibody (green, magnification ×100). (b) Quantification of cells with LC3-II dots. (c) Representative blots of autophagy-related proteins. GB further increases the ratio of LC3-II/LC3-I (d) and Beclin-1 protein expression (e) and decreases the P62 protein (f) in H9c2 cells with Ang II. Cells were pretreated with the autophagy inhibitor, 3-MA (1 μM). , vs. the control group; ^#, ^##, and ^### vs. the Ang II group. (g) 3-MA significantly attenuates Ginkgolide B-induced inhibition in ANP mRNA expression. vs. the GB group; ^## vs. the 3-MA group.

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