Patients with Coronary Artery Disease Have Lower Levels of Antibody to Heat-Stressed Fibroblast Derived Proteins, versus Normal Subjects

Figure 2

SDS-PAGE and 2-DE pattern of heat-stressed fibroblasts derived proteins. (a) The single protein band with a relative molecular weight of 70 kDa in silver-stained 12% SDS-PAGE which was carried out with two different samples (s1 and s2) prepared from heat-stressed fibroblast supernatants (lanes 2 and 4). This band was not detectable in the culture supernatant of nonstressed cells (lane 3, negative control). Lane 1 is the molecular weight size marker. (b) 2-DE gel of heat-stressed fibroblasts derived protein with Coomassie Blue staining. The arrow shows two protein spots with a relative molecular weight of 70 kDa in the acidic region of the gel.

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