Cardioprotective Effect of Nec-1 in Rats Subjected to MI/R: Downregulation of Autophagy-Like Cell Death

Figure 4

Detection and analysis of correlation between expression of MAP-LC3β in infarct zone and serum CK levels at different time points (12 h, 24 h, 48 h, and 72 h) during MI/R. (a) Representative immunoblot of LC3β protein in infarct zone (the tissue of the infarct zone is pale) of vehicle and Nec-1-treated rats at different time points (12 h, 24 h, 48 h, and 72 h) during MI/R. (b) Expression of LC3β protein in the infarct zone of vehicle and Nec-1-treated rats at different time points (12 h, 24 h, 48 h, and 72 h) during MI/R. Each column represents the ratio of the gray value of LC3β to IOD (actin). , Nec-1 group vs. vehicle group. ^##, Nec-1 group (R12h) vs. Nec-1 group (R24h). (c) Measurement of serum CK levels on rats subjected to 12 h, 24 h, 48 h, and 72 h reperfusion after myocardial ischemia for 30 min. Comparing the difference between the Nec-1 group and the vehicle group at different time points. Each column represents . , Nec-1 group vs. vehicle group. (d) Relationship between LC3β immunoreactivity (% control) and serum CK levels (U/L) at different time points (12 h, 24 h, 48 h, and 72 h) during MI/R. Analysis of correlation shows that LC3β immunoreactivity was highly correlated with serum CK levels (, ). All data were expressed as , mice for baseline; for the vehicle group: I30min , R12h , R24h , R48h , R72h ; for the Nec-1 group: I30min , R12h , R24h , R48h , R72h .

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