Knockdown of lncRNA XIST Ameliorates IL-1β-Induced Apoptosis of HUVECs and Change of Tissue Factor Level via miR-103a-3p/HMGB1 Axis in Deep Venous Thrombosis by Regulating the ROS/NF-κB Signaling Pathway

Figure 3

The expression of HMGB1 sponged by miR-103a-3p was improved by adding lncRNA XIST to sponge miR-103a-3p. (a) Bioinformatic analysis using ENCORI database shows the interaction of miR-103a-3p with HMGB1, and the binding relationship of miR-103a-3p and HMGB1 was proved by luciferase reporter gene assays. (b) The expression of miR-103a-3p in transfected HUVECs was detected by qRT-PCR. (c) The expression of HMGB1 in cotransfected cells was analyzed by qRT-PCR. (d) The expression level of HMGB1 protein in cotransfected cells was analyzed by western blot. (e) The expression level of HMGB1 protein in cotransfected cells was detected by immunofluorescence analysis (scale bar: 20 μm). The nontrasfected HUVECs served as the blank group. and .

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