Research Article

Knockdown of lncRNA XIST Ameliorates IL-1β-Induced Apoptosis of HUVECs and Change of Tissue Factor Level via miR-103a-3p/HMGB1 Axis in Deep Venous Thrombosis by Regulating the ROS/NF-κB Signaling Pathway

Figure 4

ROS-induced apoptosis and change of TF level based on adding IL-1β were regulated via lncRNA XIST/miR-103a-3p/HMGB1. (a) The ROS production in cotransfected cells was analyzed by DCFH-DA staining. (b) The expression levels of proteins related to the NF-κB pathway and apoptosis in cotransfected cells were analyzed by western blot. (c) The viability of cotransfected cells was determined by MTT assay. (d) The apoptosis of cotransfected cells was tested by Annexin V-FITC/PI double staining kit. (e) The concentration of TF antigen in cotransfected cells was determined by the ELISA kit. and .