Research Article

hsa_circWDR37_016 Regulates Hypoxia-Induced Proliferation of Pulmonary Arterial Smooth Muscle Cells

Figure 2

Effects of circWDR37 knockdown on hPASMCs proliferation under hypoxic conditions. The hPASMCs were transfected with scrambled RNA (NC) or si-circWDR37 (Si) under hypoxic conditions. (a) hPASMC viability was measured by the CCK-8 kit. (b) EdU incorporation was performed to verify the proliferation of hPASMCs. (c, d) Ki67 staining was performed by immunofluorescence (red, top panel); DAPI showed the nucleus (blue, middle panel); and merged image of Ki67 and DAPI (bottom panel). (-6 individual experiments; at least 6-10 fields from each group were photographed and scored in a blinded fashion). Comparisons of data were acquired by one-way ANOVA followed by Bonferroni post hoc.