Research Article

hsa_circWDR37_016 Regulates Hypoxia-Induced Proliferation of Pulmonary Arterial Smooth Muscle Cells

Figure 4

Effects of circWDR37 knockdown on cell migration and apoptosis in vitro. The hPASMCs were transfected with scrambled RNA (NC) or si-circWDR37 (Si) under hypoxic conditions. (a, b) The cellular migration rate of hPASMCs in different groups was analyzed by wound healing assay. (c, d) The cell apoptotic ability of hPASMCs in different groups was analyzed by TUNEL assay. Green fluorescence represents apoptotic cells, blue fluorescence represents the cell nucleus, and the bottom panel represents the merged images. (-6 individual experiments; at least 6-10 fields from each group were photographed and scored in a blinded fashion). Comparisons of data were acquired by one-way ANOVA followed by Bonferroni post hoc.
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