hsa_circWDR37_016 Regulates Hypoxia-Induced Proliferation of Pulmonary Arterial Smooth Muscle Cells

<div>Validation of miRs for circWDR37 sponging. circWDR37 was validated to be a miR-138 sponge via luciferase assays. (a) Structure diagram of luciferase reporter of circWDR37 and its mutant. Seed match regions circWDR37 and miRs were red font and indicated as vertical lines. The mutation sites in circWDR37 were indicated in blue font. (b, c) Luciferase assay for circWDR37. miR-328/miR-138 mimics was cotransfected with wild-type circWDR37 (WT) or its mutations (circWDR37-M1, M1)/(circWDR37-M2, M2) into 293 cells. Luciferase activities were measured after one-day culture. <svg height="13.532pt" id="M27" style="vertical-align:-3.4295pt" version="1.1" viewbox="-0.0498162 -10.1025 68.0304 13.532" width="68.0304pt" xmlns="http://www.w3.org/2000/svg" xmlns:xlink="http://www.w3.org/1999/xlink"><g transform="matrix(.0091,0,0,-0.0091,0,-5.741)"><path d="M486 158C486 177 478 202 466 220C413 228 386 236 336 262C386 288 413 297 466 304C478 323 486 347 485 366C470 376 444 381 422 380C389 338 368 319 321 288C323 345 329 372 349 422C339 442 322 461 305 470C289 461 271 442 262 422C281 372 287 345 290 288C243 319 222 338 189 380C167 381 142 376 125 366C125 347 133 322 145 304C198 296 225 288 275 262C225 236 198 227 145 220C133 201 125 177 126 158C141 148 167 143 189 144C222 186 243 205 290 236C288 179 282 152 262 102C272 82 289 63 306 54C322 63 340 82 350 102C330 152 324 179 321 236C368 205 390 186 422 144C444 143 470 148 486 158Z"></path></g><g transform="matrix(.0091,0,0,-0.0091,5.56,-5.741)"><path d="M486 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Comparisons of data were acquired by one-way ANOVA followed by Bonferroni post hoc.</div>

Cardiovascular Therapeutics

fig6

Figure 6

Figure 6: hsa_circWDR37_016 Regulates Hypoxia-Induced Proliferation of Pulmonary Arterial Smooth Muscle Cells 