Research Article

hsa_circWDR37_016 Regulates Hypoxia-Induced Proliferation of Pulmonary Arterial Smooth Muscle Cells

Figure 6

Validation of miRs for circWDR37 sponging. circWDR37 was validated to be a miR-138 sponge via luciferase assays. (a) Structure diagram of luciferase reporter of circWDR37 and its mutant. Seed match regions circWDR37 and miRs were red font and indicated as vertical lines. The mutation sites in circWDR37 were indicated in blue font. (b, c) Luciferase assay for circWDR37. miR-328/miR-138 mimics was cotransfected with wild-type circWDR37 (WT) or its mutations (circWDR37-M1, M1)/(circWDR37-M2, M2) into 293 cells. Luciferase activities were measured after one-day culture. ( individual experiments). Comparisons of data were acquired by one-way ANOVA followed by Bonferroni post hoc.