MIR22HG Aggravates Oxygen-Glucose Deprivation and Reoxygenation-Induced Cardiomyocyte Injury through the miR-9-3p/SH2B3 Axis

Figure 5

MIR22HG overexpression reverses the effect of SH2B3 knockdown in OGD/R-treated HL-1 cells. (a) The knockdown efficiency of sh-SH2B3 was examined by RT-qPCR (). (b) The overexpression efficiency of pcDNA3.1/MIR22HG was tested by RT-qPCR (). (c) The protein level of SH2B3 in each group was measured by western blotting (). (d) The cell viability in each group was assessed by CCK-8 assay (). (e) The cell apoptosis in each group was determined by flow cytometry analysis (). (f–i) The protein levels of Bax, Bcl-2, collagen I, fibronectin, and collagen III in OGD/R-treated HL-1 cells transfected with different vectors were measured by western blotting analysis (). , , .

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