Research Article
Valsartan Attenuated Homocysteine-Induced Impaired Autophagy and ER Stress in Human Umbilical Vein Endothelial Cells
Figure 3
Valsartan prevented Hcy-induced apoptosis in HUVECs. (a, b) Representative immunoblots and corresponding densitometry analysis of Bax, Bcl2, and cleaved-caspase 3 protein in Hcy-treated HUVECs with or without valsartan. (c) Representative photomicrographs of TUNEL staining the HUVECs in CTL, valsartan, Hcy, and Hcy+valsartan groups. (d, e) Apoptosis was detected by flow cytometry using Annexin V-FITC/PI apoptosis detection kit in Hcy-treated HUVECs with valsartan. (f, g) Representative immunoblots and corresponding densitometry analysis of Bax, Bcl2, and cleaved-caspase 3 protein in Hcy pretreated HUVECs with or without valsartan. Data are shown as ; compared with the CTL group; # compared with the Hcy group.
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