[Retracted] Nonalcoholic Fatty Liver Hepatocyte-Derived lncRNA MALAT1 Aggravates Pancreatic Cell Inflammation via the Inhibition of Autophagy by Upregulating YAP

Figure 3

NAFLD-derived exosomes activate the Hippo-YAP pathway, suppress autophagy and inflammatory responses in pancreatic cells via MALAT1. (a) qRT-PCR was used to analyze MALAT1 levels in Human hepatocytes HepG2 following si-NC and si-MALAT1 transfection. (b) qRT-PCR was used to analyze MALAT1 levels in exosomes. (c) qRT-PCR analysis of MALAT1 expression in AR42J pancreatic cells after co-culture with NAFLD-derived exosomes. (d, e) Hippo-YAP pathway-associated protein LATS1, YAP and phosphorylated YAP were detected using western blot assay. (f, g) Autophagy-associated proteins LC3II and LC3I were measured by western blot assay. (h) ELISA assay was performed on the secretion levels of IL-6 and TNF-α. vs. si-NC group/NC group/Control group; and vs. FFA group.

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