The Drosha-Independent MicroRNA6778-5p/GSK3β Axis Mediates the Proliferation of Gastric Cancer Cells

Figure 4

The miR6778-5p/GSK3β axis mediates the proliferation of Drosha low-expressing gastric cancer cells. (a) miR6778-5p acts on the 275–281 sites in the UTR of GSK3β mRNA through its seed sequence. (b) The mRNA and protein expression levels of GSK3β were detected by qRT-PCR or WB in MGC-803/Drosha KD or SGC-7901/Drosha KD cell lines after being transfected with miR6778-5p mimics ( ). Immunoblotting analyses were performed with the indicated antibodies. (c) The mRNA and protein expression levels of GSK3β were determined by qRT-PCR or WB in MGC-803/Drosha KD or SGC-7901/Drosha KD cell lines after being transfected with miR6778-5p inhibitor (). Immunoblotting analyses were performed with the indicated antibodies.

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