[Retracted] LncRNA BC083743 Silencing Exacerbated Osteoporosis by Regulating the miR-103-3p/SATB2 Axis to Inhibit Osteogenic Differentiation

Figure 4

SATB2 was a target of miR-103-3p. (a) The TargetScan website predicts that SATB2 may be a potential target for miR-103-3p. (b) Dual-luciferase reporter gene analysis indicates that miR-103-3p binds directly to the 3’UTR of SATB2. (c) Levels of SATB2 were detected by QRT-PCR after miR-103-3p overexpression or knockdown. (d) QRT-PCR was used to detect serum SATB2 levels in OP patients and normal controls. (e) QRT-PCR detects SATB2 levels in hBMSCs as osteogenic differentiation time increases. miR-103-3p inhibitors and si-SATB2 were cotransfected with hBMSCs, and ((f), (g)) QRT-PCR detects miR-103-3p and SATB2 expression. (h) QRT-PCR to detect the expression levels of osteogenic genes Runx2, OPG, and BMP2. (i) Alkaline phosphatase (ALP) activity assay for ALP activity. (j) ARS assay for the mineralization-forming ability of hBMSCs. , , ns , compared with NC mimics/NC inhibitors/NC.

(a)

(b)

(c)

(d)

(e)

(f)

(g)

(h)

(i)

(j)