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Canadian Journal of Gastroenterology
Volume 12, Issue 3, Pages 181-185
Original Article

Comparison of Activity of 10 Antibiotics Against Clinical Strains of Helicobacter pylori by Three Different Techniques

K Weiss, M Laverdiere, and C Restieri

Department of Microbiology and Infectious Diseases, Hôpital Maisonneuve-Rosemont, University of Montreal, Montréal, Québec, Canada

Received 12 November 1997; Accepted 20 January 1998

Copyright © 1998 Hindawi Publishing Corporation. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


The authors determined the susceptibility of 55 single clinical strains of Helicobacter pylori isolated in the Montreal area to 10 antibiotics by three different methods - an agar dilution technique considered to be the gold standard, a disk diffusion method and the E-test. Testing was performed on Mueller-Hinton agar supplemented with 10% sheep blood; plates were incubated at 37°C for 72 h in a microaerophilic atmosphere. The metronidazole resistance rate is about 11% in the Montreal area. Macrolides are very active against H pylori isolates, with few variations in activity between older and newer molecules. Correlation among different methods was not as good as reported in the literature for metronidazole.