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Canadian Journal of Gastroenterology and Hepatology
Volume 2016 (2016), Article ID 9607054, 9 pages
http://dx.doi.org/10.1155/2016/9607054
Research Article

VEGF Polymorphisms Related to Higher Serum Levels of Protein Identify Patients with Hepatocellular Carcinoma

1Research Centre for Biochemistry and Molecular Biology, Medical School of São José do Rio Preto (FAMERP), São José do Rio Preto, SP, Brazil
2Hepatology Unit, HB University Medical Centre, FUNFARME/FAMERP, São José do Rio Preto, SP, Brazil
3Liver Transplantation Unit, HB University Medical Centre, FUNFARME/FAMERP, São José do Rio Preto, SP, Brazil

Received 18 February 2016; Revised 10 July 2016; Accepted 4 August 2016

Academic Editor: Aldo J. Montano-Loza

Copyright © 2016 Maria Eduarda Lopes Baitello et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Hepatocellular carcinoma (HCC) is the most common primary neoplasia of the liver. Major risk factors for hepatocellular carcinoma include chronic liver diseases, carcinogenic agents, and genetic alterations as well as vascular endothelial growth factor (VEGF) involved in angiogenesis process. The aim of this study was to evaluate the association of VEGF-A (C936T and A1154G) with HCC and cirrhosis, in addition to serum levels of VEGF, clinical profile, lifestyle habits, and comorbidities. A total of 346 individuals were studied: 102 with HCC (G1), 117 with cirrhosis (G2), and 127 controls (G3). Polymorphisms were analysed by PCR/RFLP and serum levels of VEGF by ELISA. Alpha error was set at 5%. The wild-type genotype of both polymorphisms prevailed (). In G1, 23% of the patients died, with no relation to genetic profile (). Increased VEGF level was observed in G1 and G3, related to the mutant allele of VEGF-C936T and VEGF-A1154G, respectively, and compared with the wild-type genotype (; , resp.) as well as G1 versus G2 and G3 for VEGF-C936T and G1 versus G2 for VEGF-A1154G ( for both). In conclusion, there is a relationship between mutant alleles of VEGF-C936T and VEGF-A1154G polymorphisms and higher VEGF level, making them potential markers for HCC.