Metabonomics Research Progress on Liver Diseases

Table 5

The main contents of metabolomic studies on HBV-induced cirrhosis.

Reference	Methods	Sample	Main findings
[36]	UPLC-LTQOrbitrap-MS	Serum	The nine metabolites differentiated A-grade from the control group, including nicotinamid e, aminoadipic acid, glutamine, tyramine, dodecenoic acid, lysophosphatidylcholine, glycol-cysteine, cysteine amino acid, and octenoic acid; three metabolites that distinguished grade A from B, including ethanolamine, glycine, glycosylchenodeoxycholic acid; 10 metabolites that differentiated grade B from C, including aminoadipic acid, taurine, aminoacetone, glycine, pyruvate, glycolcholodeoxycholic acid, alanine, pipecolic acid, methionine, and serine.
[37]	RP-HPLC-QTOF-MS and HILIC-QTOF-MS	Serum	Oleic acid, bilirubin, acetylcarnitine, and GCDCA were significantly increased in cirrhosis patients and distinguished cirrhosis from control.
[38]	GC/MS	Serum	Acetic acid, sorbitol, D-lactic acid, hexanoic acid, 1-naph-thalenamine, butanoic acid, phosphoric acid, D-glucitol, and glucose were the strongest segregation between cirrhosis and CHB.
[39]	^1H-NMR	Serum	Compared with the compensatory period, some metabolites increased significantly, including glucose, citrate, succinate, phenylalanine, tyrosine, lysine, glutamine, and creatine, whereas some decreased notably, namely, LDL, VLDL, N-acyl glycoprotein (NAG), choline, acetone, isoleucine, and valine in the decompensation period.

UPLC-LTQOrbitrap-MS: ultraperformance liquid chromatography coupled with linear trap quadrupole Orbitrap mass spectrometry.
RP-HPLC-QTOF-MS and HILIC-QTOF-MS: reversed-phased (RP) high liquid chromatography and hydrophilic interaction chromatography (HILIC) coupled with quadrupole time-of-flight mass spectrometry.