Notch Signaling Pathway Is Inhibited in the Development of Barrett’s Esophagus: An In Vivo and In Vitro Study

Figure 5

Effects of hydrochloric acid on Notch1, Hes1, K13, and MUC2 expressions. Cells were treated with different concentrations of hydrochloric acid (pH4, pH5, and pH6) for 24 h, 48 h, 72 h, and 96 h, respectively. (a–d) Notch1, Hes1, K13, and MUC2 mRNA expression levels in Het-1A cells exposing to different concentrations of hydrochloric acid for different time periods. , statistically significant difference. , statistically significant difference. , statistically significant difference. (e) Representative western blot results of Notch1, Hes1, K13, and MUC2 protein expressions in Het-1A cells treated with hydrochloric acid (pH5) for different time periods.

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