Notch Signaling Pathway Is Inhibited in the Development of Barrett’s Esophagus: An In Vivo and In Vitro Study

Figure 7

Effects of mixture of hydrochloric acid and deoxycholic acid on Notch1, Hes1, K13, and MUC2 expressions. Cells were treated with mixture of hydrochloric acid (pH = 5) and deoxycholic acid (500 umol/L) for 24 h, 48 h, 72 h, and 96 h, respectively. (a) Notch1, Hes1, K13, and MUC2 mRNA expression levels in Het-1A cells exposing to mixture of hydrochloric acid and deoxycholic acid for different time periods. , statistically significant difference. , statistically significant difference. , statistically significant difference. (b) Representative western blot results of Notch1, Hes1, K13, and MUC2 protein expressions in Het-1A cells treated with mixture of hydrochloric acid (pH = 5) and deoxycholic acid (500 umol/L) for different time periods.

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