β-Catenin Regulation in Sporadic Colorectal Carcinogenesis: Not as Simple as APC

Table 1

Forward and reverse sequences of primers for all genes and their annealing temperatures.

Gene of interest	Forward sequence	Reverse sequence	Annealing temp (°C)
-catenin	5′-CTGACTTTGCTTGCTTGA-3	5′-CACTATAACTTAACACTACGAG-3′	60
COX-2	5′-GAAGCCAATTCAGTAGGT-3′	5′-ACGAAGTGATGAGAAGAC-3′	57
PPAR	5′-AGGTTTGCTGAATGTGAAG-3′	5′-AATCTGTCTGAGGTCTGTC-3′	60
IL-17A	5′-CCACACTCCCCAAAGCAGTT-3′	5′-TGACATGCCATTCCTCAGGG-3′	53
Reference genes	Forward sequence	Reverse sequence	Annealing temp (°C)
ALUSq	5′-CATGGTGAAACCCCGTCTCTA-3	5′-GCCTCAGCCTCCCGAGTAG-3′	60
ALUSx	5′-TGGTGAAACCCCGTCTCTACTAA-3′	5′-CCTCAGCCTCCCGAGTAGCT-3′	60
SF3A1	Commercial primer, obtained from GeNorm™ Normalization kit. (Primer Design, UK)		60