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Figure 7: Real-time PCR analysis shown as fold change of mRNA expression of (a) Hepc, (b) Tf, (c) Hjv, and (d) FPN-1 in the liver of the rats after administration of N. oleander. Significant upregulation in fold change was noted up to 12 h time point. Results were normalized with housekeeping gene ß-actin. (statistically significant changes are marked with asterisks (, , and ; mean ± SEM; ).