N-3 Polyunsaturated Fatty Acids Stimulate Bile Acid Detoxification in Human Cell Models

Figure 2

Eicosapentaenoic and docosahexaenoic acids modulate bile acid secretion by human hepatoma HepG2 cells. Cells were treated with DMSO (vehicle, 0.1% v/v), 25 or 50 μM eicosapentaenoic (EPA, E), and/or docosahexaenoic (DHA, D) acids for 24 H. Bile acid levels in culture media were measured using LC-MS/MS as described in the materials and methods section. Total (a), tauro- (b), glyco- (c), and unconjugated (d) BAs were calculated based on the measurements of individual BAs (e). The results (mean ± SD) are representative of two independent experiments. Statistically significant differences were analyzed using one-way analysis of variance (ANOVA) (). n.d: not detected. BA: bile acid; CA: cholic acid; CDCA: chenodeoxycholic acid; DCA: deoxycholic acid; G: glyco-; HCA: hyocholic acid; HDCA: hyodeoxycholic acid; LCA: lithocholic acid; UDCA: ursodeoxycholic acid; -S: sulfate; T: tauro-.

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