Colon Epithelial MicroRNA Network in Fatty Liver

Figure 2

Transfection of MetS-FL miR mimics into Caco-2 colon epithelial monolayers leads to miR-specific changes in barrier integrity and epithelial cell junction. (a, b, c) Transepithelial electrical resistance (TEER) was measured daily pre- and posttransfection of cells with control miR mimic or MetS-FL miR mimics. Background TEER measurements in “blank” wells without cells were subtracted from TEER values in transfected cells. The subtracted TEER values are normalized against seeding surface area and expressed as Ohm-cm². Data are means ± standard deviations calculated from at least four biological replicates. Bar graphs show TEER values of untransfected cells and cells transfected with control C.elegans cel-miR-67 mimic or each of the MetS-FL miR mimics. indicates significant (p <0.05) changes in barrier TEER in miR mimic-transfected compared to nontransfected cells. (d) Transfection of MetS-FL miR mimics into Caco-2 colon epithelial monolayers leads to altered appearance of Zona Occludens-1 (ZO-1) and Occludin within the cell junction. Images show immunofluorescence staining and confocal microscopy visualization of ZO-1 (red), Occludin (green), or both (yellow). Scale bars are 10 μm.

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