Research Article
The Prevalence of Helicobacter Pylori babA, homB, aspA, and sabA Genes and Its Relationship with Clinical Outcomes in Turkey
Table 1
Oligonucleotide primers and sizes of the PCR products for virulence factors of H. pylori.
(a) |
| | Primer | Primer sequence | Annealing Temperature (°C) | Primer Length | Product Size (bp) |
| | AspA-F | ATGCGTATGGAGCATGATTTCATT | 55.9 | 24 | 1401 | | AspA-R | TTTATGCTTTTTGAAAGCGTGAGGGCTT | 62.2 | 28 | |
| | BabA-F | ATGAAAAAACACATCCTTTCATTA | 52.5 | 24 | 2192 | | BabA-R | TTATTCAAATACACGCTATAGAGTCTT | 57.4 | 27 | |
| | CagA-F | AATACACCAACGCCTCCAAG | 57.3 | 20 | 1741 | | CagA-R | GCTGACAAAGGAGCACTTCC | 59.4 | 20 | |
| | HomB-F | TACAGACGCTCAAGGCAATG | 57.3 | 20 | 1005 | | HomB-R | TCTATGGGTAGGGCGTTTTG | 57.3 | 20 | |
| | SabA-F | CTCTCTCTCGCTTGCGGTAT | 59.4 | 20 | 187 | | SabA-R | TTGAATGCTTTGCCTCAATG | 53.2 | 20 | |
| | VacA-F | ACAAACACACCGCAAAATCA | 53.2 | 20 | 1624 | | VacA-F | AACGGCCACATTAGTGGAAG | 57.3 | 20 | |
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(b) |
| | Gender | Kind of disease | Number of patients | AspA+ | BabA+ | CagA+ | HomB+ | SabA+ | VacA+ | Mean Age |
| | Male | NS | 21 | 16 | 3 | 2 | 1 | 2 | 4 | 39.85±17.11 | | | GU | 20 | 15 | 6 | 0 | 0 | 1 | 6 | 49.4±17.82 | | Female | NS | 25 | 18 | 5 | 1 | 1 | 3 | 4 | 47.52±17.01 | | | GU | 16 | 9 | 3 | 0 | 1 | 0 | 6 | 45.93±12.28 |
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(c) |
| | | NS | GU | Total | p value |
| | Number of patients | 46 | 36 | 82 | - | | AspA | 34 (73.91%) | 25 (69.44%) | 59 (71.95%) | <0.05 | | BabA | 7 (15.21%) | 9 (25%) | 16 (19.51%) | ns | | CagA | 3 (6.52%) | 0 (0%) | 3 (3.65%) | ns | | HomB | 2 (4.34%) | 1 (2.77%) | 3 (3.65%) | ns | | SabA | 5 (10.86%) | 1 (2.77%) | 6 (7.31%) | ns | | VacA | 12 (26.08%) | 8 (22.22%) | 20 (24.39%) | ns |
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