Review Article
Systematic Review and Meta-Analysis of the Utility of Circular RNAs as Biomarkers of Hepatocellular Carcinoma
Table 2
Main characteristics of 10 studies included in diagnostic meta-analysis.
| | Author, year | Country | Sample size (case/control) | Male (case) | HBsAg (case) | Tumor size (≤5) | Control | Profile | Specimen type | Method | Reference gene | Sensitivity (%) | Specificity (%) |
| | Zhang et al. 2017 | China | 104/52 | 87 | NA | NA | healthy people | hsa_circ_0001445 | plasma | qRT-PCR
(SYBR) | GAPDH | 94.20 | 71.20 | | | | 104/57 | 87 | NA | NA | liver cirrhosis | hsa_circ_0001445 | plasma | qRT-PCR
(SYBR) | GAPDH | 74.00 | 54.40 | | | | 104/44 | 87 | NA | NA | chronic hepatitis B | hsa_circ_0001445 | plasma | qRT-PCR
(SYBR) | GAPDH | 69.20 | 72.70 | | Qin et al. 2016 | China | 89/89 | 74 | 66 | 50 | adjacent non-tumor tissues | hsa_circ_0001649 | tissue | qRT-PCR
(SYBR) | β-actin | 81.00 | 69.00 | | Zhang et al. 2017 | China | 102/152 | 90 | 86 | 62 | adjacent non-tumor tissues and chronic hepatitis | hsa_circ_0004018 | tissue | qRT-PCR
(SYBR) | GAPDH | 71.60 | 81.50 | | Chen et al. 2018 | China | 78/78 | 70 | 65 | NA | adjacent non-tumor tissues | hsa_circ_0128298 | tissue | qRT-PCR
(SYBR) | GAPDH | 67.40 | 80.50 | | Yao et al. 2017 | China | 102/102 | 87 | 85 | 29 | adjacent non-tumor tissues | cirZKSCAN1 | tissue | qRT-PCR
(SYBR) | GAPDH | 82.20 | 72.40 | | Shang et al. 2016 | China | 30/30 | 25 | 23 | 19 | adjacent non-tumor tissues | hsa_circ_0005075 | tissue | qRT-PCR
(SYBR) | GAPDH | 83.30 | 90.00 | | Fu et al. 2017 | China | 107/137 | 96 | 90 | 62 | chronic hepatitis and liver cirrhosis | hsa_circ_0003570 | tissue | qRT-PCR
(SYBR) | GAPDH | 44.90 | 86.80 | | Yao et al. 2018 | China | 100/70 | 14 | NA | 61 | chronic hepatitis | hsa_circ_0068669 | tissue | qRT-PCR
(SYBR) | GAPDH | 59.00 | 71.00 |
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HBsAg: surface antigen of the hepatitis B virus; qRT-PCR: quantitative reverse transcription PCR; NA: not available.
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